In comparison to BB the use of BB-2ßME led to a shift in the electrophoretic protein pattern of CPE in which some of the higher molecular weight bands reduce in intensity (white double arrow – Fig. 2) and others disappear. The pattern of protein bands obtained with SB is similar to that obtained with BB. However, SB solubilized better the higher and lower molecular weight bands. The immunoblot reactivity showed that the greatest reactivity with sera of animals immunized by CPE-BB-2βME and CPE-TB/HCl to their corresponding extract (Fig. 3).