Genetic diversity of microsatellite DNA
The mean number of alleles per locus across nine loci in regions of Florida was 18.9. The mean observed heterozygosity was 0.688, while the mean expected heterozygosity was higher at a value of 0.859 (Table 1). After Bonferroni correction for multiple comparisons across all regions, four of the nine loci showed deviations from expectations under HWE (Table 1). However, by region and locus, only four HWE tests were significant after Bonferroni corrections and no loci deviated in more than two regions (Table 1). Therefore, we retained all nine loci for downstream analyses. There were no cases of linkage disequilibrium detected for any pair of loci (raw genotypic data for microsatellites are available in Table S2).