Microorganism and growth media
Methylomicrobium alcaliphilum 20ZR was provided by Dr. Marina Kalyuzhnaya, San Diego State University, and Synechococcus sp. PCC7002 was provided by Dr. Alexander Beliaev, Pacific Northwest National Lab. M. alcaliphilum 20ZR cells were grown in modified P-medium.Synechococcus sp. PCC7002 cells were grown in A+ medium. For the coculture, the growth media consisted of 10% P-medium and 90% A+ medium.
Methylococcus capsulatuswas acquired from the American Type Culture Collection (ATCC 33009), andChlorella sorokiniana (UTEX 2805) was acquired from UTEX Culture Collection of Algae. M. capsulatus Bath cells were grown in NMS medium. C. sorokiniana cells were grown in N8 medium. For the coculture, the growth media consisted of 10% NMS medium and 90% N8 medium.