Immunofluorescence analyses of immune cell infiltration of the spinal cord in EAE mice
C57Bl6/J mice were immunized with MOG and treated with vehicle or sapropterin (BH4) starting at the day of immunization. The tissue was prepared 19 days after immunization. Myeloid cells were stained with anti CD11b, Iba1 and F4/80. T-cells were identified via anti-CD3. NeuN was used as neuronal counterstain, and DAPI to label nuclei. For the overviews, tiled images were captured and stitched. Scale bars are 500 µm, and 20 µm (zoom in).

Figure 5: