Effects of sapropterin medication on the disease severity in EAE mice
A: Time courses of the clinical EAE scores (median ± siqr), body weights (mean ± SD), score frequency distribution and AUCs of score of SJL/J mice in PLP-induced relapsing-remitting EAE. Mice were treated orally with vehicle (2% DMSO), sapropterin (2 mg/d) or DAHP (4 mg/d) in the drinking water starting at the day of immunization. The score courses were analyzed using Friedmann statistics, the score frequency distribution using the ChiSquare test and the AUCs of the scores were compared with the Kruskal Wallis test (n = 6-8 per group).
B: Time courses of the clinical EAE scores, body weights, score frequency distribution and AUCs of EAE scores in C57Bl6/J mice in MOG-induced primary progressive EAE. Mice were treated orally with vehicle or sapropterin soaked cornflakes once daily (dosages as in A). The score courses were analyzed using Wilcoxon P statistics, the score frequency distribution using the ChiSquare test and the AUCs of the scores were compared with the Mann Whitney U test (n = 10 per group).
The box plots in A and B show the interquartile range, the line is the median, whiskers show minimum to maximum, each scatter is a mouse.

Figure 3: