Immunofluorescence analyses of immune cell infiltration of the
spinal cord in EAE mice
C57Bl6/J mice were immunized with MOG and treated with vehicle or
sapropterin (BH4) starting at the day of immunization. The tissue was
prepared 19 days after immunization. Myeloid cells were stained with
anti CD11b, Iba1 and F4/80. T-cells were identified via anti-CD3. NeuN
was used as neuronal counterstain, and DAPI to label nuclei. For the
overviews, tiled images were captured and stitched. Scale bars are 500
µm, and 20 µm (zoom in).
Figure 5: