FACS analyses of immune cells in the spinal cord of EAE mice
A: FACS analysis of lumbar spinal cord cells from vehicle or
sapropterin (BH4) treated C57Bl6/J-EAE mice. The tissue was dissected 19
days after immunization and 300000 cells were counted of each seven
mice. The dot plot shows exemplary cell clouds gated according to
forward scatter (FSC) versus sideward scatter (SSC). The gates show
lymphocytes and monocytes according to size and granularity. The bar
chart shows the frequency of lymphocytes as percentage of viable cells
(compared per unpaired, 2-sided t-test; *P<0.05). CD4 and CD8
positive T-cells subpopulations are shown in the right panel histograms.
B: FACS analysis of lumbar spinal cord cells as in A. Immune
cells were further analyzed for F4/80 and CD36 (exemplary dot plot,
middle). The box plot shows the frequency of myeloid subpopulations (as
percentage of all immune cells). The box is the interquartile range,
whiskers show minimum to maximum, the line is the median. Each scatter
is one mouse.
Figure 4: