Figure 5. Metabolite purification and detection for NMR analysis.
Panel (A) is an extracted ion chromatogram of IVM, M1, and M3 during the preparation chromatography, and UV chromatogram at 240nm for the trapping experiment (the dotted lines indicate the start and end of the trapping procedure, (B) is a mass spectrum from the peaks in the extracted ion chromatogram showed that the molecules form stable sodium adducts.