Fig. 6. Effect of conformation-selective photolabeling by
azido-riluzole on gating kinetics of F1579A mutant channels.
A, B, C Assessment of gating kinetics and equilibrium before
and after azido-riluzole perfusion and UV irradiation. Protocols and
color of lines are the same as in Fig. 5: Control (black), after
resting-state-illumination (blue); after V-half-illumination (teal);
after inactivated-state-illumination (green). Amplitudes were normalized
to the maximal amplitude in control. Insets: Amplitudes normalized each
to its own maxima.
Fig. 7. Riluzole does not block the pore. Riluzole was docked
into the structural model of human Nav1.4 (PDB ID: 6AGF) (A )
and its F1586A mutant (B ). Gray sticks: best poses of riluzole;
sticks and balls: F436 (teal), F1284 (blue), and F1586 (red); green,
cyan, orange, and marine: domains I, II, III, and IV.