Fig. 6. Effect of conformation-selective photolabeling by azido-riluzole on gating kinetics of F1579A mutant channels.
A, B, C Assessment of gating kinetics and equilibrium before and after azido-riluzole perfusion and UV irradiation. Protocols and color of lines are the same as in Fig. 5: Control (black), after resting-state-illumination (blue); after V-half-illumination (teal); after inactivated-state-illumination (green). Amplitudes were normalized to the maximal amplitude in control. Insets: Amplitudes normalized each to its own maxima.
Fig. 7. Riluzole does not block the pore. Riluzole was docked into the structural model of human Nav1.4 (PDB ID: 6AGF) (A ) and its F1586A mutant (B ). Gray sticks: best poses of riluzole; sticks and balls: F436 (teal), F1284 (blue), and F1586 (red); green, cyan, orange, and marine: domains I, II, III, and IV.