Description of inoculums prepared for soil spiking.
In experiments 1-3, whole blood was collected from wild boar experimentally infected with ASFV “Armenia08”. These animal trials were previously conducted for pathogenesis studies. The blood was mixed for 15-20 minutes with glass beads to remove fibrin. The blood was then stored at -80°C until use. Since experiments were completed at different time points, stocks for spiking the soil matrices had different titers, but differed no more than one log in considered volumes. Infected blood from experiment 1 had a titer of 7.25 log10 50% hemadsorbing doses (HAD50) per mL, blood from experiment 2 had a titer of 6.00 log10 HAD50/mL and blood from experiment 3 had a titer of 7.00 log10HAD50/mL.
ASFV-Kenya1033ΔCD2vdsRed was derived from ASFV-Kenya1033 as described by Hübner et al. (2018) by substitution of the CD2v ORF (EP402R) from codon 77 to the translational stop codon (386) by a dsRed expression cassette.