Conclusion
This study elucidates the continuous spread and emergence ASFV genotype I TRS variants in pig farming areas in Nigeria within three years of the study. Although phylogeny revealed genotype I, however, six variants were recovered following sequence analysis of CVR of B602L gene. This study presents the first report of three of the variants that are co-circulating in four States in Nigeria. The increasing recovery of new variants of genotype I in Nigeria should be a source of serious concern, particularly for a stable DNA virus like ASFV. But the epidemiological implications of the findings are unknown and need further investigation.