Morphological identification
Morphological identification was mainly classified in situ by visual inspection, then detailed morphological identification and classification was in laboratory by stereomicroscope microscopic examination. All the collected prawn species were taxonomically classified according to Liu (1955), Liang (2004) and Li et al. (2007) mainly based on the distinguishing morphological characters of the male collected specimens(X. Li, Liu, Liang, & Chen, 2007 ; Liang, 2004 ; Liu, 1955 ).
According to the morphological identification results, multiple representative individuals of per taxonomic group were selected for abdominal muscle sampling, and the sampling process should as careful as possible to avoid damaging the complete shape of the specimen. The obtained tissue samples were immediately stored in 95% ethanol and numbered for DNA extraction (While ensuring the specimen coverage of each species, individuals with moderate body size should be selected as far as possible for EP tube preservation and numbering; the larger individuals were marked by winding coils).