Database search
In general, our morphological identification results matched the BLASTN
annotations of the NCBI and BOLD databases, with at least 97%
identities. According to the identification results of Species Level
Barcode Records of the BOLD reference sequence library, theP.clarkii , N.denticulate , N.davidi ,C.gracilipes , M.maculatum andM.nipponensehave been identified to the species level. The identification results
were relatively reliable, which can basically be judged as effective
identification species. The identities of Macrobrachium sp.
’qilianensis’ and P.modestus are all greater than 98%, but the
search results show that the sequence identities between theP.modestus and the three
unpublishedM.sp.’qilianensis’in the library is also high sequence identities (even sometimes they has
the highest identities). At the same time, in the retrieval ofM.sp. ’qilianensis’ , the identities of itself and two unpublishedP.modestus was also relatively high. After verification, the
aboveM.sp.’qilianensis’ (Accession: FJ958200,
FJ958201) was sourced from GenBank which was direct submission and
unpublished
by
Cheng (2009)(Chen, Tsai, & Tzeng,
2009 ). However, there was no corresponding morphological description
and species identification of M.sp.’qilianensis’ was found, so
the M.sp.’qilianensis’ may be a controversial species. In
addition, the search results of the G.pisinnus are only
96%-98%, the identities is slightly lower; the search results ofP.sinensis show no corresponding records. The search results of
Species Level Barcode Records are similar to the search results of
Species Level Barcode Records, which will not be repeated here; the NCBI
search shows a trend similar to the BOLD result, while the same species
sequence has been retrieved from P.sinensis (MK994929, MK994930).