Tree- based approach
A large number of tests show that PTP is superior to GMYC on simulated data and its results are comparable to GMYC on real data sets meanwhile PTP requires less for data sets and requires only a simple phylogenetic tree (Zhang, Kapli, Pavlidis, & Stamatakis, 2013 ). Therefore, in this study, we chose PTP analysis to assist in species definition. PTP can delimit species based on the Phylogenetic Species Concept. So the entities output by PTP are in theory species. Bayesian Poisson tree process (bPTP) analyses were run on the web server (https://species.h-its.org/ptp) with 100,000 MCMC generations, a burn- in of 0.1 and other parameters as default. The ML tree generated by RAXML is selected as the input data(Stamatakis, 2006 ).