Morphological identification
Morphological identification was mainly classified in situ by visual
inspection, then detailed morphological identification and
classification was in laboratory by stereomicroscope microscopic
examination. All the collected prawn species were taxonomically
classified according to Liu (1955), Liang (2004) and Li et al. (2007)
mainly based on the distinguishing morphological characters of the male
collected specimens(X. Li, Liu,
Liang, & Chen, 2007 ; Liang,
2004 ; Liu, 1955 ).
According to the morphological identification results, multiple
representative individuals of per taxonomic group were selected for
abdominal muscle sampling, and the sampling process should as careful as
possible to avoid damaging the complete shape of the specimen. The
obtained tissue samples were immediately stored in 95% ethanol and
numbered for DNA extraction (While ensuring the specimen coverage of
each species, individuals with moderate body size should be selected as
far as possible for EP tube preservation and numbering; the larger
individuals were marked by winding coils).