Sample collection
According to the distribution of water systems in Henan Province, a
total of 46 sampling sites were covered in this survey for collecting
freshwater shrimp (Fig.1). The sample points cover the main streams or
some tributaries of the four major rivers of the province (Table S1). In
this study, about 1200 samples from 11 species, 6 genera and 4 families
of Amphipoda and Decapoda were collected. As many individuals per
species as possible were obtained for this study. However, in some
cases, only one few individuals per region per species could be
collected. Most of the prawns were collected by the shrimp traps, but a
lot of individuals were captured by market buying. The collections were
preserved in 95% ethanol for subsequent morphological observation and
molecular identification, part of the samples was fixed in 10%
formalin, and all samples were stored in the Fisheries College of Henan
Normal University.