Enrichment of key immune, pruritus and barrier-related pathways
We used gene set variation analysis (GSVA) on the previously published AD transcriptome (MADAD), and Th1-, Th2- and Th17/Th22- as well as pruritus and barrier-related genes, to evaluate how the two approaches compare.29,42,68 We observed similar significant enrichment in all key analyses in both biopsies and tape-strips analyses (Figure 5 ).