FIGURES LEGEND :
Figure 1. Study summary. (A) Study design. (B) Venn diagram of
differentially expressed genes (DEGs) in tape-strip and biopsy groups by
fold-change (|FCH|)>2 and false discovery
rate (FDR)<0.05. Up-regulated (red ) and down-regulated
(blue ) DEGs in AD versus control in each group depict shared
versus unique genes across the two techniques.
Figure 2. Heatmap of immune-related genes. Heatmap of the top
differentially expressed immune genes in tape-stripped (A), and biopsied
(B) AD lesional skin. Fold-change
(|FCH|)>2 and false discovery rate
(FDR)<0.05. Tables show fold-changes in lesional AD versus
normal skin. World Cloud diagram of immune DEGs of tape-strips (C) and
biopsy (D) group. The size of the letters represents the magnitude of
the FCHs in AD versus healthy skin. LS, Lesional.
***FDR<0.001, **FDR<0.01, *FDR<0.05,
+FDR<0.1.
Figure 3. Heatmap of pruritus-relates genes. Heatmap of the
differentially expressed pruritus-related genes in tape-stripped (A),
and biopsied (B) AD lesional skin. Fold-change
(|FCH|)>2 and false discovery rate
(FDR)<0.05. Tables show fold-changes in lesional AD versus
normal skin. LS, Lesional. ***FDR<0.001, **FDR<0.01,
*FDR<0.05, +FDR<0.1.
Figure 4. Heatmap of barrier-related genes. Heatmap of the
differentially expressed barrier-related genes in tape-stripped (A), and
biopsied (B) AD lesional skin. Fold-change
(|FCH|)>2 and false discovery rate
(FDR)<0.05.Tables show fold-changes in lesional AD versus
normal skin. LS, Lesional. ***FDR<0.001, **FDR<0.01,
*FDR<0.05, +FDR<0.1.
Figure 5. Gene-set variation analysis (GSVA). Gene-set
variation analyses (GSVA) of immune-related and barrier-related genes.
Red bars represent means. Black symbols : significance of
comparison to normal; red symbols: significance of comparison
between lesional and non-lesional skin. MADAD, Meta-Analysis Derived AD
transcriptome; LS, Lesional. ***P <0.001,
**P <0.01, *P <0.05,
+P <0.1.