Extraction and cultivation of BALB/c mice bone marrow
derived dendritic cell (DC)
BALB/c mice were sacrificed, then bone marrow cells were extracted and
made into single cell suspension. After red blood cells having been
cracked, cell suspension was cultivated according to
106/ml concentration. Thereafter, rmGM-CSF (20 ng/ml)
and rmIL-4 (20 ng/ml) were added. On the sixth day, morphology of DCs
was observed by phase contrast microscope and the expressive rate of
CD11c, CD86 and MHC-II were detected by flow cytometry.