Downregulating levels of CD3ζ and CD62L in CTL by MDSC
Respectively, the levels of CD3ζ and CD62L in CTL were detected and compared by reai-time PCR (Fig. 3A) and western-blot analysis (Fig. 3B). In reai-time PCR test, significant difference of CD3ζ (F = 20.315, P = 0.002 < 0.05) and CD62L (F = 13.858,P = 0.006 < 0.05) occurred between CTL group, CTL+MDSC group and CTL+MDSC+DOX group. Western-blot analysis showed the same results. Significant difference of CD3ζ (F = 28.241, P = 0.001 < 0.05) and CD62L (F = 41.142, P< 0.001) were also seen among the three groups.