Separation and purification of MDSC in vitro
BALB/c mice were sacrificed, then bone marrow cells were extracted and
made into single cell suspension. After red blood cells having been
cracked, cell suspension was administrated by anti-Gr-1 monoclonal Ab
(mAb), anti-CD11b mAb, anti-CD80 mAb, anti-F4/80 mAb, anti-CD11c mAb and
anti-MHCII mAb. Differentiation of MDSC and proportion of
Gr-1+CD11b+MDSCs were detected by
flow cytometry.
Bone marrow cells were suspended by PBS and MDSC were separated by
Percoll Density Gradient Centrifugation. Then, cell suspension was
collected and separated by CD11b magnetic bead and purity of
Gr-1+CD11b+MDSC was detected by flow
cytometry.