MDSC regulating the killing effect of neuroblastoma
antigen-specific CTL in vitro
CTL, CTL+MDSC (1:4), and CTL+MDSC(1:4)+DOX(2µmol/L) were mixed with
SK-N-SH cells and incubated respectively (CTL:SK-N-SH = 20:1). At the
same time, blank control group, target cells group and effector cells
group were set up. CCK-8 was added into the cell system (20 μl/hole)
after incubation and absorbance value (A value) was detected in 450 nm
wavelength by enzyme standard instrument. The cytotoxic activities of
effector cells were evaluated by the killing rate. The killing rate of
effector cells was calculated as follow, kill rate (%) = [A value of
target cells group - (A value of experimental group - A value of
effector cells group)]/A value of target cells group × 100%. The
killing rates of CTLs to SK-N-SH cells between the groups were compared.
The secretion levels of IL-2 and IFN-γ in the supernatant between the
groups were detected and compared by enzyme-linked immunosorbent assay
(ELISA).