Extraction and cultivation of BALB/c mice bone marrow derived dendritic cell (DC)
BALB/c mice were sacrificed, then bone marrow cells were extracted and made into single cell suspension. After red blood cells having been cracked, cell suspension was cultivated according to 106/ml concentration. Thereafter, rmGM-CSF (20 ng/ml) and rmIL-4 (20 ng/ml) were added. On the sixth day, morphology of DCs was observed by phase contrast microscope and the expressive rate of CD11c, CD86 and MHC-II were detected by flow cytometry.