3.5 Association between donor CSF3R SNPs and patient survival
In univariate analysis, we found that rs3917980 and rs2275472 polymorphisms in donors were associated with the LFS and overall survival of recipients (Table 3), while none of the other CSF3R SNPs in donors were associated with LFS or patient survival. Although CSF3R SNPs had statistically significant trends with LFS and OS, the biological results of LFS and OS associated with these the rs3917980 and rs2275472 genotypes were completely opposite. Patients receiving stem cells from donors with the rs3917980G/G or A/G genotype trended towards increased LFS (25.5% versus 13.6%, p=.016) and OS rates (p=.044) (Fig 3A and 3B), while those with the rs2275472T/C genotype trended towards LFS rates (p=.066) (Fig 3C and 3D). Further subgroup analyses based on HLA loci showed no relations between CSF3R SNPs and LFS and OS in HLA-matched groups (p>.05); however, patients receiving stem cells from HLA-mismatched donors with the rs3917980G/G or A/G genotype showed better LFS and a trend towards a higher OS rate (p=.015 and p=.098, respectively) (Fig 3E and 3F). Notably, no association was found between the rs2275472T/C genotype and OS in total HLA-unmatched recipients (p=0.42), and patients receiving stem cells from HLA-unmatched donors with the rs2275472T/C genotype had no associations with incidence of LFS rates and OS(p>.05) (data not shown).
The Cox regression analysis showed that no significance was found for any CSF3R SNPs for predicting relapse, TRM, LFS or OS (p>.05). The engraftment of PLTs was a protective factor for LFS and OS (p<.001, Table 3)
Table 3 Univariate analysis for clinical outcomes of allogeneic stem cell transplantation