The rs3917980 genotype was associated with better LFS and OS in patients post allo-HSCT
As rs3917980 and rs2275472 SNPs showed a completely opposite effect on clinical prognosis after transplantation in univariate analysis and approximately 33% of the healthy donors carried two SNPs, we speculated whether the biological effects caused by the above SNPs would be weaker than those caused by a single SNP. Then, we divided all patients into the following four groups according to whether their corresponding donors contained two SNPs: rs3917980G/G or A/G genotype single-positive group, rs2275472 T/C genotype single-positive group, double-positive group and double-negative group. Because the number of positive patients was only 4 out of 273, we created a group with double-negative SNPs. Cumulative analysis of LFS and OS showed that recipients receiving grafts from healthy donors with the rs3917980 G/G or A/G genotype had higher LFS rates than those who received grafts from healthy donors with the rs22754272 T/C genotype (p=.037) (Fig 4A), while the LFS and OS rates of the wild-type genotype in the group with double-negative SNPs were between the values of the above groups, further proving that the rs3917980 G/G or A/G genotype may be a strong protective factor for a better LFS; whoever no significant differences were found among the groups (p=.14) (Fig 4B).