2.2 Sequencing of CSFR single nucleotide polymorphisms (SNPs)
Sequencing was performed using the Ion Torrent platform. CSF3R gene exon region and exon-intro junction region primers were designed by Ampliseq design. The Ion Ampliseq Library Kit, 2.0(Thermo Fisher) was used for library construction. The Ion PGM Hi-Q OT2 Kit and Hi-Q Sequencing 200 Kit reagents were used to perform sequencing. Then, sequencing BAM files and variant caller files were acquired. We then performed data screening and judged the data quality based on mutation frequency, sequencing quality value, forward and reverse sequencing depth and chain bias. Then, SNP sites were screened by population frequency, mutation type and mutation function.