Chromosome, Fluorescence In Situ Hybridization and Molecular
Analysis
Bone marrow samples was obtained for routine cytogenetic and molecular
analysis. Commercially avaliable routine reverse transcriptase
polymerase chain reaction (RT-PCR) and flourescence in situ
hybridiziation (FISH) panels were used prognostic classification.
Reccurent genetic abnormalities for acute leukemia including t (4:11), t
(9:22) and t (12:21) were negative. Convantional cytogenetic analysis
was performed from over night colchisine exposed cell cultures. 46,XX,
t(5;15)(p15;q11.2)[3]/46,XX[17] karyotype was found in the
analysis (Figure 2). After that results, chromosome analysis was
performed from peripheral blood samples to exclude constitutional
changes. The result was normal. The bone marrow cytogenetic result
revealed as a rare and somatic abberation.