METHODS
Animals Male Wistar rats (n=270) FMRP-USP, Ribeirao Preto, Brazil; 200–250 g body weight) were housed and maintained in groups of three per cage in a controlled environment under a light-dark cycle of 12 h (lights on at 06:00 and off at 18:00 h), in a quiet room with controlled temperature (23 ºC ± 1 ºC). All experimental animal procedures were approved by the local Animal Care and Use Committee of the University of São Paulo/Brazil at the Ribeirao Preto Campus (2016.1.667.58.4) and were conducted in accordance with the Guide for the Care and Use of Laboratory Animals of the National Council for the Control of Animal Experimentation (CONCEA).