Experiment 2- Effect of chronic doxycycline on the development of LID
To extend these novel findings, we also examined whether chronic, doxycycline administration would attenuate the development of LID. We examined whether the co-treatment of doxycycline (40 mg kg-1, i.p., once a day, 15 days) to lesioned rats, 30 min before L-DOPA, influenced the development of LID. The experimental groups consisted of 6-OHDA+chronic-vehicle+L-DOPA (n=11) and 6-OHDA+chronic-doxycycline+L-DOPA (n=11). There was no AIMs manifestation in the control group animals [no-lesion+chronic-vehicle+vehicle (n=6); 6-OHDA+chronic-vehicle+vehicle (n=6); 6-OHDA+chronic-doxycycline+vehicle (n=7)]. Individually all animals were submitted to the open field test one hour after L-DOPA treatment to evaluate their motor behavior. Comparisons of the scores between the experimental groups were performed by two-way ANOVA-RM, followed by Bonferroni’s multiple comparisons test.