Experiment 2- Effect of chronic doxycycline on the
development of LID
To extend these novel findings, we also examined whether chronic,
doxycycline administration would attenuate the development of LID. We
examined whether the co-treatment of doxycycline (40 mg
kg-1, i.p., once a day, 15 days) to lesioned rats, 30
min before L-DOPA, influenced the development of LID. The experimental
groups consisted of 6-OHDA+chronic-vehicle+L-DOPA (n=11) and
6-OHDA+chronic-doxycycline+L-DOPA (n=11). There was no AIMs
manifestation in the control group animals
[no-lesion+chronic-vehicle+vehicle (n=6);
6-OHDA+chronic-vehicle+vehicle (n=6); 6-OHDA+chronic-doxycycline+vehicle
(n=7)]. Individually all animals were submitted to the open field test
one hour after L-DOPA treatment to evaluate their motor behavior.
Comparisons of the scores between the experimental groups were performed
by two-way ANOVA-RM, followed by Bonferroni’s multiple comparisons test.