METHODS
Animals Male Wistar rats (n=270) FMRP-USP, Ribeirao
Preto, Brazil; 200–250 g body weight) were housed and maintained in
groups of three per cage in a controlled environment under a light-dark
cycle of 12 h (lights on at 06:00 and off at 18:00 h), in a quiet room
with controlled temperature (23 ºC ± 1 ºC). All experimental animal
procedures were approved by the local Animal Care and Use Committee of
the University of São Paulo/Brazil at the Ribeirao Preto Campus
(2016.1.667.58.4) and were conducted in accordance with the Guide for
the Care and Use of Laboratory Animals of the National Council for the
Control of Animal Experimentation (CONCEA).