Impact of acute administration of doxycycline on LID expression
once dyskinesia was present
During the chronic L-DOPA treatment, 90% of the rats developed AIMs
affecting the contralateral forelimb and orofacial structures, together
with trunk dystonia (Fig. 1-A, p<0.05, 6-OHDA+vehicle+L-DOPA).
The maximum total AIMs score (ALO-AIMs Fig. 1-A) was reached 40–80 min
after L-DOPA administration and gradually declined to the baseline level
over 180 min (Fig. 1-B). Administration of doxycycline (40 mg kg-1), 30
min before L-DOPA significantly reduced AIMs (89%; Fig. 1-A,
6-OHDA+vehicle+L-DOPA ALO AIMs score=92.22±8.66 vs.
6-OHDA+doxycyclineL-DOPA ALO AIMs score=10.56±4.89, p<0.0001)
over 20 to 120 min observation period (Fig. 1-B). Acute doxycycline
before L-DOPA also decreased the individual AIM categories (axial,
forelimb, and orofacial; Supplementary Fig. 2). AIM was not observed in
both the non-lesioned rats and lesioned rats treated with vehicle or
doxycycline (data not shown).