HCV-genotyping and genotyping for TLR3.rs3775290 (c.1377C/T) SNP
PCR-RFLP was used for HCV genotyping after reverse transcription as
described [15].
PCR-RFLP
was, also, used for the identification of the TLR3.rs3775290 (c.1377
C/T) gene SNPs. PCR was conducted in a total of 25 μl reaction volume
with 20 pmol of each of the forward and reverse primers as described
[16].