3.1 Virus detection, identification, isolation and growth
kinetics
VESV, VSV, SVDV, FMDV and SVV were detected by reverse transcription
polymerase chain reaction (RT-PCR). During our analysis, we identified a
product of approximately 850bp that was cloned by RT-PCR (Fig.
S1) . This amplicon was sequenced and confirmed to be part of the
nucleic acid sequence of SVV. Virus isolation was performed in a BHK-21
cell line; cytopathic effect (CPE) was observed in the third passage(Fig. S2) . Indirect fluorescence assay (IFA) results showed
that when treated with anti-SVV VP1-mouse serum, infected cells showed
obvious green fluorescence (Fig. 1A) . One-step growth curves
were obtained after infecting BHK-21 cells with CH-GX-01-2019; analysis
showed that the maximal viral titers of CH-GX-01-2019 were approximately
107.0 TCID50/ml (Fig. 1B) .