Toxoplasma gondii in Neotropical Primates
Carmen Niehaus1,2, Manuel Spínola3, Chunlei Su4, Norman Rojas5, Oscar Rico-Chávez6, Carlos N. Ibarra-Cerdeña7, Janet Foley8, Gerardo Suzán6, Gustavo A. Gutiérrez-Espeleta1, Andrea Chaves1,8,*.
1Escuela de Biología, Universidad de Costa Rica.
2Posgrado Regional en Ciencias Veterinarias Tropicales, Universidad Nacional de Costa Rica.
3Instituto Internacional de Conservación y Manejo de Vida Silvestre, Universidad Nacional de Costa Rica.
4Department of Microbiology, College of Arts and Sciences, University of Tennessee, Knoxville.
5Centro de Investigación en Enfermedades Tropicales, Facultad de Microbiología, Universidad de Costa Rica.
6Departamento de Etología, Fauna Silvestre y Animales de Laboratorio, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México.
7Departamento de Ecología Humana, Centro de Investigaciones y de Estudios de Avanzados del IPN (Cinvestav), Unidad Mérida, Mérida.
8Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California, Davis.
*Corresponding author: Andrea Chaves
E-mail address: andreachaves.biol@gmail.com
Summary:
The apicomplexan parasite Toxoplasma gondii has been found in more than 350 species of homoeothermic vertebrates in diverse climates and geographic areas. In most animals, T. gondii produces mild or asymptomatic infection. However, acute and hyperacute toxoplasmosis is associated with high mortality rates observed in Neotropical primates (NP) in captivity. NP are distributed in 20 countries across the Americas, and although infection has been reported in certain countries and species, toxoplasmosis in the wild and its impact on NP population survival is unknown. Differences among species in exposure rates and disease susceptibility may be due in part to differences in host behavior and ecology. Costa Rica has four species of NP, howler (Alouatta palliata ), spider (Ateles geoffroyi ), capuchin (Cebus imitator ), and squirrel monkeys (Saimiri oerstedii ). Here we report for the first time NP exposure to T. gondii using a modified agglutination test (MAT) in 245 serum samples of NP (198 wild and 47 from captivity) from Costa Rica. Associations of serostatus with environmental (forest cover, annual mean temperature), anthropogenic (human population density), and biological (sex) variables in howler and capuchin monkeys were evaluated. The seroprevalence among wild NP was 11.6% (23/198), compared with 60% (28/47) in captive monkeys, with significant differences between species (P <0.05), suggesting behavior and ecology influences. In general, antibody titers were low for wild NP (<128) and high for captive NP (>8192), suggesting higher exposure due to management factors and increased life span in captivity. Seropositivity in howler monkeys was positively related to forest cover and inversely related to annual rainfall. For capuchins, annual rainfall was inversely related to seropositivity. Surveillance of T. gondii in NP in captivity and in the wild is required to understand drivers of the infection and develop novel strategies to protect them.
Keywords:
Serology, Alouatta palliata , Ateles geoffroyi , Cebus imitator , Saimiri oerstedii, endoparasites, Latin America