3.5 CHO-S Cell Growth Analysis
CHO-S cells were cultured for 72 hours in three culture vessels:
Standard 96-well plate (Control), Redbud Labs Microplate (No Mixing) and
Redbud Labs Microplate (Mixing @ 6000 rpm). There was an observed
increase in cell growth when cultured in an agitated Redbud Labs vessel
(566,000 cells/mL) compared to both the unmixed Redbud Labs vessel
(499,214 cells/mL) and the standard 96-well plate (432,071 cells/mL)
(Figure 5A). As established, vessels agitated with Redbud Labs
microposts have increased oxygen transport capabilities, thus it is
expected that cell growth would be increased in such vessels. Further,
there was an observed increase in cell growth in both vessels containing
Redbud posts compared to the standard 96-well plate, suggesting post
microenvironment promotes increase cell growth.