3.6 T Cell Growth Analysis
CD4+ T cells were expanded under two different concentrations of essential interleukins, IL-2, IL-7, and IL-15 (000 or 110) which corresponded to low and high concentrations of each respectively and were either mixed via surface attached microposts or unmixed. CD4+ T cells were seeded at a concentration of 166,667 cells/mL and harvested 7 days later. The expansion kinetics for T cells are shown in Figure 5B. There was an observed peak cell yield on day 5 for all conditions, indicating the need for culture feed and potential further activation. When mixed, the 110 condition experienced the highest cell yield on day 5 (117,500 cells/mL) whereas the 000 condition had a lower day 5 cell yield (45,000 cells/mL). When unmixed, the 110 condition had the highest cell yield on day 5 (72,500 cells/mL) whereas the 000 condition had a lower day 5 cell yield (45,000 cells/mL). When mixed, both interleukin concentration sets (000 & 110) had higher daily cell yield than their respective unmixed conditions, until day 7, which saw a decline in all conditions.