3.6 T Cell Growth Analysis
CD4+ T cells were expanded under two different
concentrations of essential interleukins, IL-2, IL-7, and IL-15 (000 or
110) which corresponded to low and high concentrations of each
respectively and were either mixed via surface attached microposts or
unmixed. CD4+ T cells were seeded at a concentration
of 166,667 cells/mL and harvested 7 days later. The expansion kinetics
for T cells are shown in Figure 5B. There was an observed peak cell
yield on day 5 for all conditions, indicating the need for culture feed
and potential further activation. When mixed, the 110 condition
experienced the highest cell yield on day 5 (117,500 cells/mL) whereas
the 000 condition had a lower day 5 cell yield (45,000 cells/mL). When
unmixed, the 110 condition had the highest cell yield on day 5 (72,500
cells/mL) whereas the 000 condition had a lower day 5 cell yield (45,000
cells/mL). When mixed, both interleukin concentration sets (000 & 110)
had higher daily cell yield than their respective unmixed conditions,
until day 7, which saw a decline in all conditions.