Figure 1. Schematic overview of mRNA and cDNA display using PUREfrex system. The system starts with a designed DNA library (randomized sequences) harboring T7 promoter and a leader sequence that is complementary to the DNA tag. DNA library is in vitrotranscribed to mRNA library and undergo ligation with the puromycin-FITC DNA tag. Tagged product is then gel purified and translated using the PUREfrex 1.0 kit. The resulting mRNA-peptide conjugate is then gel purified. The purified product is then either used directly in the binding step (mRNA display) or first reverse transcribed to become an mRNA/cDNA-peptide conjugate (cDNA display). Both products are used for binding against anti-FLAG M2 magnetic beads and the selected conjugates are further reverse transcribed and sequenced using the Illumina MiSeq system. Preparation time (hours) for each step is shown in red with exception of Miseq sequencing (5 days) carried out only after all samples were obtained.