Objective: Analyse the relationship between COVID-19 with IL-10, IL-1β, IL-6, MCP-1, TNF-α, IP-10 and IL-4. Methods: The serum levels of cytokines above were detected by ELISA. Results: Most of the serum level of cytokines above in patients with COVID-19 were significantly higher than those in healthy people, and some of them were significantly higher than those in patients with other diseases. AUC of COVID-19 diagnosed by they were 0.735, 0.775, 0.595, 0.821, 0.848, 0.387 and 0.987. The serum levels of some cytokines in male patients had noticeably higher than those in female patients. The serum level of almost all cytokines of the elderly was higher than that of the youth and middle-aged patients. The serum level of IP-10 in patients with COVID-19 was positively correlated with IgM, while TNF-α was negatively correlated with IgG. The levels of cytokines in patients were higher at 25 days after onset than at 20 days, TNF-α and IL-4 were significantly lower at 30 days than at 25 days, while IP-10 were reversed. Conclusion: The detection of cytokines have some clinical value for diagnosis of COVID-19, and that of IL-4 and TNF-α was the best. The levels of cytokines in patients have some relationships with gender, age and course of disease.