Basophil reactivity is reduced over time29.
|
Perform BAT within a few hours (up to 24h) of blood collection
|
Good transportation system between sites to ensure timely delivery of
samples.
Test samples of all site within the same time frame.
|
Basophil reactivity can be affected by vibration and changes in
temperature29.
|
Ensure method of transportation that ensure stability of temperature
transfer of samples.
|
Prefer transport system with temperature control for samples.
|
Immunosupressors, including oral corticosteroids, can reduce basophil
response30.
|
Avoid immunosupressors before blood collection for BAT.
|
Need to continue treatment with immunosupressors should be an
exclusion criteria of studies using BAT.
|
Exposure to allergen, chronic inflammation and infection can induce
basophil degranulation and homing to the
tissues101.
|
Avoid performing BAT after allergen exposure or during infection or
chronic inflammatory condition.
|
Blood for BAT needs to be collected prior to allergen exposure (namely
challenge but not SPT).
Active infections inflammatory conditions should be an exclusion
criteria of studies using BAT.
|
Basophil activation can vary with the anticoagulant
used29. |
BAT can be performed in blood collected into
heparin or EDTA. |
Blood for BAT should be collected using the same
material and methodology during studies and between
sites. |
Measurement of basophil activation can be influenced by the markers used
to identify the basophils, by the BAT protocol and by flow
cytometry4. |
BAT should be performed with a validated
method and optimised conditions. |
The same reagents and protocol should
be used throughout a clinical trial and flow cytometers should be
standardized. |
Quantification of basophil activation can vary with the method adopted
for data analyses4,32. |
Criteria should be defined
for each step of data analyses. Automated data analyses can be
considered. |
The exact same methodology needs to be used between
centers and throughout the clinical trial. |