Sampling and sequencing
Daphnia populations were collected from 13 lakes in the South
Island and 1 lake in the North Island, NZ in March and October 2019
(Fig. 1). In each location, Daphnia were sampled by tows of a
conical plankton net and preserved in DNA/RNA Shield. DNA from each
pooled population sample (50-200 individuals) was extracted with Zymo
DNA Clean and Concentrator-25 Kit and sequenced on an Illumina HiSeq
2500 platform.