Statistics
Data are expressed as mean ± standard error of the mean (SEM). Normality distribution was tested with Shapiro-Wilk test. Two tailed Student’s test was used to compare two groups, one-way ANOVA was used to compare one variable in multiple groups and two-way ANOVA to compare two variables. Both ANOVA were followed by the corresponding post hoc analyses test. To analyze no-normal distribution data a non-parametric Mann-Whitney or Kruskal-Wallis tests were used. Survival data were analyzed using the log-rank test and aneurysm incidence by the Fisher exact test. GraphPad Prism 8.0 (GrahPad Software, San Diego California USA) was used to perform the statistical analysis. Values of p <0.05 were considered statistically significant.