Retention Mapping Assessment
Retention mapping was conducted with mAb-1 for all resins using 1 mL
HiTrap columns with a GE Healthcare ÄKTA™ avant 25 system. Protein was
loaded with a 4 min residence time, and protein concentration was
monitored through A280 measurements. The feed material (purified
antibody) was used as the absorbance at 100% breakthrough.