Figure 2: Interaction networks of the diurnal proteome.
An association network analysis using STRING-DB (https://string-db.org/)
of statistically significant diurnally changing proteins was performed
using the generated unsupervised clusters shown in Figure 1. Edge
thickness indicates confidence of the connection between two nodes
(0.5 - 1.0). Changing proteins (grey circles) are labeled by either
their primary gene annotation or Arabidopsis gene identifier (AGI). The
colored outline of each node represents the in silico predicted
subcellular localization of this protein (SUBAcon;
suba3.plantenergy.uwa.edu.au). Nucleus (red), cytosol (orange), plastid
(green), mitochondria (blue), plasma membrane (purple), peroxisome
(dark yellow), endoplasmic reticulum/golgi/secreted (black) are
depicted. A second layer of STRING-DB identified proteins (white nodes)
not found in each respective significantly changing protein cluster was
used to highlight the interconnectedness of proteins in the cluster.
Multiple nodes encompassed by a labelled grey circle represent proteins
involved in the same cellular process.