Figure 2: Interaction networks of the diurnal proteome.
An association network analysis using STRING-DB (https://string-db.org/) of statistically significant diurnally changing proteins was performed using the generated unsupervised clusters shown in Figure 1. Edge thickness indicates confidence of the connection between two nodes (0.5 - 1.0). Changing proteins (grey circles) are labeled by either their primary gene annotation or Arabidopsis gene identifier (AGI). The colored outline of each node represents the in silico predicted subcellular localization of this protein (SUBAcon; suba3.plantenergy.uwa.edu.au). Nucleus (red), cytosol (orange), plastid (green), mitochondria (blue), plasma membrane (purple), peroxisome (dark yellow), endoplasmic reticulum/golgi/secreted (black) are depicted. A second layer of STRING-DB identified proteins (white nodes) not found in each respective significantly changing protein cluster was used to highlight the interconnectedness of proteins in the cluster. Multiple nodes encompassed by a labelled grey circle represent proteins involved in the same cellular process.