Supplemental Figure 2: Hierarchical heatmap of significantly changing diurnal phosphopeptides at the D-L transition.
The hierarchical heatmap was generated using the R package Pheatmap and Euclidean distance. Standardized relative log2 FC in phosphopeptide abundance is shown along with the corresponding AGI and phosphopeptide with phosphorylation site probabilities. GO terms of proteins in the heatmap clusters are shown on the right together with their predicted subcellular localization (SUBAcon). The segments of the circles represent the nucleus (red), cytosol (orange), plastid (green), mitochondria (blue), plasma membrane (purple) and other (black) localizations. The numbers below each pie chart represent the unique protein identifications. The time points of sampling for phosphoprotein analysis were 30 min before light (BL30), 10 min after light (AL10) and 30 min after light (AL30).