Figure 4: Interaction networks of the diurnal phosphoproteome at the D-L and L-D transitions.
An association network analysis of statistically significant diurnally changing phosphorylated proteins was performed using the STRING-DB (ANOVA P value ≤ 0.05). Edge thickness indicates strength of the connection between two nodes (0.5 - 1.0). Phosphorylated proteins (grey circles) are labeled by either their primary gene annotation or Arabidopsis gene identifier (AGI). Outer circle around each node depicts the standardized relative log2 FC in phosphorylation status of this protein between time-points. The sliding scale of yellow to blue represents a relative increase and decrease in phosphorylation, respectively. The inner colored circles represent in silicopredicted subcellular localization (SUBAcon; suba3.plantenergy.uwa.edu.au). Nucleus (red), cytosol (orange), plastid (green), mitochondria (blue), plasma membrane (purple), peroxisome (dark yellow), endoplasmic reticulum/golgi/secreted (black) are depicted. A second shell of 5 STING-DB proteins (white circles) not found in our dataset was used to highlight the interconnectedness of the network. Multiple nodes encompassed by a labelled grey circle represent proteins involved in the same cellular process.