Figure 1: Analysis of the diurnal proteome: clustering,
enrichment analysis and subcellular localization.
(A) Significantly changing proteins (FC ≥ 1.5, ANOVA P value ≤ 0.05, ≥ 2
peptides) were subjected to an unsupervised clustering analysis (GProX;
http://gprox.sourceforge.net) resolving 6 protein clusters. Y- and
X-axis depict standardized expression level and harvest time (Zeitgeber
time; ZT), respectively. Median expression is depicted in blue. (B) Term
enrichment analysis of significantly changing proteins using SetRank
(P value ≤ 0.01, size ≥ 2). (C) Standardized diurnal transcript
expression level of each corresponding clustered protein (Log10). Median
expression is depicted in blue. Transcript expression level was obtained
from Diurnal DB (http://diurnal.mocklerlab.org/). (D) In
silico subcellular localization analysis of significantly changing
proteins using SUBAcon (SUBA3; http://suba3.plantenergy.uwa.edu.au).
Bracketed numbers represent the number of proteins per cluster.