3.5 BIN analysis
In the present study, 1227 records generated 201 different BINs. In particular, 117 BINs were not recorded previously in the BOLD database and most sequences representing new endemic species received new BIN assignments. The Diptera speciesChironomus sp. XJ andC. heterodentatus shared the same BIN [AAW4009], P. bullum shared a BIN assignment [ACB4789] with P. laetum. BIN Discordance analysis was performed on BOLD (February 2018), and the results showed that two BINs were discordant with our prior taxonomic assignments, which was attributed to haplotype sharing and low interspecific divergence. A number of 141 BIN clusters were found to be taxonomically concordant with other barcode data on BOLD, that is, they were assigned to the same species. A number of 58 records are singletons, implying that these BINs were represented by only one sequence. Nine species were assigned to a total of 21 BINs (Table S2).