Primers Sequence (5’ to 3’) Purpose
P1 tgaactttatctgagaatagtcaatcttcggaaatcccaggtggc For the construction of multimer of insertion construct (LF-AbR-GOI-RF)
P2 catttttcttcctccctttcttatcataatacataattttcaaactg
P3 cagtttgaaaattatgtattatgataagaaagggaggaagaaaaatg
P4 gccacctgggatttccgaagattgactattctcagataaagttca
P5 cagcgcaaatgctcccgctatcatcgagctccagcatccttgcagtcttcatatg
P6 catatgaagactgcaaggatgctggagctcgatgatagcgggagcatttgcgctg
P7 tttggaaagcgaggga For the construction of variant T47C
P8 ctgaacgccatcgtaaagattgacgttaacgcaaacaacaaacttatc
P9 gataagtttgttgtttgcgttaacgtcaatctttacgatggcgttcag
P10 cgttggttgtatccgtgt
P11 cgcacctgcggtgctgcagcctgagcagacatgttgctgaacgcc For the construction of variant G81T
P12 ggcgttcagcaacatgtctgctcaggctgcagcaccgcaggtgcg
P13 gcggcggacttgccgtcgatgtcgagctgggtcgtgacgctggggtcgtc For the construction of variant T806A
P14 gacgaccccagcgtcacgacccagctcgacatcgacggcaagtccgccgc
P15 gccttcttgcgctccaccgcgacggacttgccgtcgatgtcgagc For the construction of variant C821T
P16 gctcgacatcgacggcaagtccgtcgcggtggagcgcaagaaggc
P17 gatgtggccgatgccggggaacgacaggtggctcgccgcgatcag For the construction of variant C892T
P18 ctgatcgcggcgagccacctgtcgttccccggcatcggccacatc
P19 gagtagttcaccggcacgaaatggtagcccttgccttcggcgc For the construction of variant G938A
P20 gcgccgaaggcaagggctaccatttcgtgccggtgaactactc