Antibody serology rapid test kit
We used a commercial HEALGEN IgG/IgM antibody rapid test kit in our
study (Zhejiang Orient Gene Biotech Co., Ltd,
China).30 HEALGEN antibody rapid test kit is a gold
immunochromatographic assay for the rapid, qualitative, and differential
detection of IgG and IgM to COVID 19 in human whole blood, plasma, or
serum. In our study, we used finger prick whole blood or blood serum as
the specimens for testing (Figure 3). We acquired serum by leaving
venous blood in a plain tube under room temperature for approximately 3
hours to coagulate before carefully pipetting the serum sample, avoiding
the buffy coat, or clotted blood (Figure 4). The amount of whole blood
and serum samples used for serology test were five and 10uL
respectively. Specimens were pipetted using the prepared plastic dropper
and transferred into the sample well on the cassette. Three drops of
buffers were added to the buffer well. The cassette was left for 10
minutes before the result was read.