Antibody serology rapid test kit
We used a commercial HEALGEN IgG/IgM antibody rapid test kit in our study (Zhejiang Orient Gene Biotech Co., Ltd, China).30 HEALGEN antibody rapid test kit is a gold immunochromatographic assay for the rapid, qualitative, and differential detection of IgG and IgM to COVID 19 in human whole blood, plasma, or serum. In our study, we used finger prick whole blood or blood serum as the specimens for testing (Figure 3). We acquired serum by leaving venous blood in a plain tube under room temperature for approximately 3 hours to coagulate before carefully pipetting the serum sample, avoiding the buffy coat, or clotted blood (Figure 4). The amount of whole blood and serum samples used for serology test were five and 10uL respectively. Specimens were pipetted using the prepared plastic dropper and transferred into the sample well on the cassette. Three drops of buffers were added to the buffer well. The cassette was left for 10 minutes before the result was read.