Resistance-associated gene sequences
In total, we collected 403 insecticide target gene sequences, including 87 AChE sequences (41 ace1 gene sequences and 46 ace2 gene sequences), 71 VGSC sequences, 71 RyR sequences, and 174nAChR sequences (containing 69 alpha1 subunit sequences, 19 alpha3 subunit sequences, 15 alpha6 subunit sequences, and 71 beta1 subunit sequences). All of these gene sequences refer to 82 insect species with ace gene sequences belonging to 54 insect species,VGSC sequences belonging to 71 insect species, RyRsequences belonging to 71 species, and nAChR sequences belonging to 74 insect species.
Amongst the 82 insect species, the genome of 71 insects have been published and have annotated OGSs. In total, we extracted 11,356 detoxification gene sequences from the OGS files of 71 insect species. For the remaining 11 species, we obtained 1,309 detoxification gene sequences by searching against NCBI nucleotide database. In total, we obtained 12,665 detoxification gene sequences, including 9,260 P450 gene sequences, 2,188 GST gene sequences, and 1,217 CCE gene sequences.