To collect target gene sequences, we downloaded confirmed the full
cDNA sequences of VGSC, AChE, RyR, and nAChR
from InsectBase (Yin et al., 2016). Next, these confirmed target gene
sequences were used as queries to BLASTP against the NCBI GenBank for
each target each species. The first search step obtained target
sequences for species of most orders. Then, we selected the obtained
target sequences from species with annotated genome as the secondary
queries to search against other species within the same order. These two
step searches yielded most sequences of four targets in the tested
species. For species still without target sequences, we used the target
sequences from the closely related species as the tertiary queries to
search against the genome of this species.
To collect detoxification gene sequences of different species as
comprehensively as possible, genome official gene set (OGS) files for
the species were downloaded. Then, we selected all the sequences
annotated as “cytochrome P450” or “glutathione S-transferase” or
“carboxyl/cholinesterase”. For some important insect species without
published genome OGSs, the detoxification gene sequences were obtained
by directly searching against NCBI nucleotide database with terms:
(((cytochrome P450) OR glutathione S-transferase) OR
carboxyl/cholinesterase) AND “species name” [Organism].