Labeling of B cells with CFSE
B cells (107cells/ml) were incubated at 37°C for 15
min with 5μl of 500μM CFSE diluted in PBS. In order to stop the
reaction, 100μl of fetal calf serum (FCS) was added to per ml of cells.
Cells were washed twice in RPMI-FCS and resuspended in AIM-V medium.