2.4. MSD immunoassay
The MSD multiplex immunoassay was used to measure the total IgE as well
as specific IgE-levels. This method is based on the U-plex protocol of
MSD (Rockville, MD, USA) [14], but was adapted specifically for
IgE-measurements, using biotinylated allergens and IgE standards from
Radim Diagnostics (Freiburg im Breisgau, Germany).