2.7. Cell culture
Mouse hepatocyte AML-12 cells were obtained from ATCC (American Type
Culture Collection, Manassas, VA, Cat# CRL-2254, RRID:CVCL_0140) and
were cultured in Dulbecco’s modified Eagle’s medium/Ham’s F-12 (GIBCO)
supplemented with 10% fetal bovine serum (FBS), a mixture of insulin,
transferrin, and selenium (ITS; Collaborative Research), and 0.1 μM
dexamethasone at 37°C in a 5% CO2 atmosphere.
Mycoplasma contamination was not detected by 16s rRNA-based mycoplasma
group-specific PCR. AML-12 cells were used between passages 15 and 16.