2.3. Biochemical parameters
The plasma glucose levels were determined using Glutest Neo Super (Sanwa
Chemical Co. Kanagawa, Japan) just before and 4 hours after the
administration of OSI-906 or the vehicle. Serum insulin levels were
determined using an insulin kit (Morinaga Institute of Biological
Science, Yokohama, Japan). The glycogen content in the liver was
determined using a Determiner-GL-E Kit (Wako Pure Chemical Industries).
The serum glutamic pyruvic transaminase (GPT), free fatty acid (FFA),
and triglyceride (TG) levels were assayed using enzymatic methods (Wako
Pure Chemical Industries).