2.2. Drugs
OSI-906 (linsitinib) was purchased from MedChem Express (NJ, USA).
Eight-week-old mice were orally administered 10 μL/g body weight of
water or linagliptin (3 mg/kg/daily, oral gavage) (provided by
Boehringer Ingelheim, Ingelheim, Germany) and were also additionally
administered 10 μL/g body weight of either the vehicle (30% Solutol
HS-15; BASF, Ludwigshafen am Rhein, Germany) or OSI-906 (45 mg/kg)
orally at 30 min after the previous administration once a day for 7
days. Liraglutide was obtained from Novo Nordisk (Bagsvaerd, Denmark).
AML-12 cells were treated with 10 nM linagliptin or 100 nM liraglutide
in the presence of 200 nM OSI-906.