Figure 3. Proteomic and phosphoproteomic analysis of the liver from OSI-906- and linagliptin-treated mice.
(a ) Protocol for proteomic and phosphoproteomic analyses using liver samples (n = 5 per group, respectively).
(b ) Volcano plot of hepatic proteomic data. Molecules identified in comparisons of vehicle vs. OSI-906 (left) and OSI-906 vs. OSI-906 + linagliptin are shown (n = 5 per group, respectively).
(c ) Venn diagram of differentially expressed molecules in OSI-906 vs. Vehicle and OSI-906 + Lina vs. OSI-906 in proteomic analyses of liver samples. The numbers of proteins that were significantly upregulated or downregulated (ANOVA P value < 0.05, fold change > 1.2) compared with the respective controls are shown (n = 5 per group, respectively). The lists of overlapping proteins are shown in Supplementary Table 1.