2.6. Immunoblotting
For immunoblotting, 40 mg of liver tissues were lysed with T-PER tissue
protein extraction reagent (Thermo Fisher Scientific) with protease
inhibitor (Nacalai Tesque) and phosphatase inhibitor (Nacalai Tesque).
After centrifugation, the extracts were subjected to immunoblotting with
antibodies to acetyl-lysine (Abcam #21623, RRID:AB_446436, 1/1000),
perilipin 2 (PROGEN #42, 1/2000), NNMT (Abcam #119758, RRID:
AB_10902083, 1/600), and glyceraldehyde-3-phosphate dehydrogenase
(GAPDH) (Cell Signaling Technology #5174, RRID: AB_10622025, 1/1000).
Densitometry was performed using Image J software.