2.2. Drugs
OSI-906 (linsitinib) was purchased from MedChem Express (NJ, USA). Eight-week-old mice were orally administered 10 μL/g body weight of water or linagliptin (3 mg/kg/daily, oral gavage) (provided by Boehringer Ingelheim, Ingelheim, Germany) and were also additionally administered 10 μL/g body weight of either the vehicle (30% Solutol HS-15; BASF, Ludwigshafen am Rhein, Germany) or OSI-906 (45 mg/kg) orally at 30 min after the previous administration once a day for 7 days. Liraglutide was obtained from Novo Nordisk (Bagsvaerd, Denmark). AML-12 cells were treated with 10 nM linagliptin or 100 nM liraglutide in the presence of 200 nM OSI-906.