Fig. 5. Lack of salt-elicited TAR in matched environments. Parental plants had been exposed to different stress intensities by soil salinity (NaCl; Mock, S-I, S-II, S-III). Salt tolerance of F1 and F2 plants was quantified by root growth reduction (%) over a 5-day period on NaCl-containing agar medium relative to the average root growth on agar medium without NaCl. Boxplots show the interquartile range (IQR; box) ± 1.5xIQR (whiskers), including median (horizonal line) and replication units (dots). (a) Unaltered tolerance of F1 plants to 50 and 100 mM NaCl. Data represent growth reduction percentages of single plants within F1 populations from similarly treated parents (n=60). ns: no statistically significant differences (ANOVA; α=0.05). Root growth data for individual F1 populations are shown in Fig. S4a; root tolerance data for individual F1 populations are shown in Fig. S4c. (b) Unaltered tolerance of F2 plants to 50 and 100 mM NaCl after one stress-free F1 generation. Data represent growth reduction percentages of single plants within F2 populations that share a common parental ancestor (n=18-20). ns: no statistically significant differences (ANOVA; α=0.05). Root growth data for individual F2 populations are shown in Fig. S4b; root tolerance data for individual F2 populations are shown in Fig. S4d.
 
Fig. 6. Costs and benefits of TAR in mismatched environments. Parental plants had been exposed to different stress severities by Pst (green), Pc (blue) or soil salinity (orange). F1 plants were tested for resistance against different stresses than the parental stress. (a) Increased Pc susceptibility in F1 progeny from Pst-exposed parents. Box plots show lesion diameters (mm) of plants within F1 populations from similarly treated parental plants (n=76-80). See legend of Fig. 4 for details. Different letters indicate statistically significant differences between parental treatments (ANOVA + Tukey post-hoc test; α=0.05). Data for individual F1 populations are shown in Fig. S5a. (b) Reduced salt tolerance in F1 progeny from Pst-exposed parents. Box plots show root growth reduction percentages by 50 mM NaCl of plants within F1 populations from similarly treated parental plants (n=40). See legend of Fig. 5 for details. Different letters indicate statistically significant differences (ANOVA + Tukey post-hoc test; α=0.05). Root growth data for individual F1 populations at 0, 50 and 100 mM NaCl are shown in Fig. S5b; tolerance data for individual F1 populations to 50 and 100 mM NaCl are shown in Fig. S5c. (c) Increased Hpa susceptibility in F1 progeny from Pc-exposed parents. Stacked bars show leaf frequency distributions across Hpa resistance classes within F1 populations from similarly treated parents (n=400-500). See legend of Fig. 3b for details. Different letters indicate statistically significant differences (pairwise Fisher’s exact tests + Bonferroni FDR, α=0.05). Data for individual F1 populations are shown in Fig. S6a. (d) Unaltered salt tolerance in F1 progeny from Pc-exposed parents. Box plots show root growth reduction percentages by 50 mM NaCl of plants within F1 populations from similarly treated parental plants (n=47). See legend of Fig. 5 for details. ns: no statistically significant differences (ANOVA; α=0.05). Root growth data for individual F1 populations at 0, 50 and 100 mM NaCl are shown in Fig. S6B; tolerance data for individual F1 populations to 50 and 100 mM NaCl are shown in Fig. S6c. (e) Non-specific TAR against Hpa in F1 progeny from NaCl-exposed parents. Stacked bars show leaf frequency distributions across Hpa resistance classes within F1 populations from similarly treated parents (n=350-800). See legend of Fig. 3b for details. Different letters indicate statistically significant differences (Pairwise Fisher’s exact tests + Bonferroni FDR, α=0.05). Data for individual F1 populations are shown in Fig. S7a. (f) Non-specific TAR against Pc in F1 progeny from NaCl-treated parents. Box plots show lesion diameters (mm) of plants within F1 populations from similarly treated parental plants (n=30-60). See legend of Fig. 4 for details. Different letters indicate statistically significant differences between parental treatments (ANOVA + Tukey post-hoc test; α=0.05). Data for individual F1 populations are shown in Fig. S7b.
  

Supplementary Figures and Table