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\begin{document}

\title{Molecular sexing of Xenarthra: A tool for genetic and ecological studies}



\author[1]{Carmen Barragan Ruiz}%
\author[1]{Eliana Paviotti-Fischer}%
\author[1]{Karen Rodriguez-Castro}%
\author[2]{Arnaud Jean Desbiez}%
\author[1]{Pedro  Galetti Jr}%
\affil[1]{Universidade Federal de São Carlos}%
\affil[2]{Institute for the Conservation of Wild Animals}%


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  \date{May 20, 2024}


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\begin{abstract}
Although sex assignment is essential to study biology and ecology of a
given animal, in Xenarthra there is still no standardized assay for
gender genetic identification. Here, we evaluate the potential of two
nuclear fragments {[}SRY (\textasciitilde{}180pb) and Zinc finger
(\textasciitilde{}400pb) genes{]} for sex identification of specimens
who have lost their morphological characteristics because of road-kills.
DNA samples from seven Xenarthra species were amplified and sequenced
for one or both segments. Finally, we performed a case study using
tissue samples from road-kill carcasses, which supported the suitability
of our markers for poor-quality DNA. The method proved to be efficient
for different types of samples and may be especially useful for studies
using road-killed and hunted animals.%
\end{abstract}%



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\textbf{Hosted file}

\verb`MS Molec_Sex with tables.doc`  available at \url{https://authorea.com/users/317169/articles/447296-molecular-sexing-of-xenarthra-a-tool-for-genetic-and-ecological-studies}

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