2.4 DNA sequencing
A total of 43 libraries were prepared at Fasteris facilities (Geneva, Switzerland) using the Metafast protocol (www.fasteris.com/metafast). These libraries were sequenced either on Illumina HiSeq 2500 (2 x 125 bp) or Illumina NextSeq (2 x 150 bp) platforms (Illumina, San Diego, CA, USA), targeting 300,000 forward and reverse reads per sample and primer pair.