(FIGURE 1 here)
The culturing result (Löwenstein-Jensen medium slant) from lung tubercles of the calf showed two strains of typical M. boviscolony morphology. Heat-killed colonies were tested by RD4-based PCR and confirmed that both strains were M. bovis . Spoligotyping11 then classified these two strains into different spoligotype patterns, SB0134 and SB0133 (FIGURE 2). Genomic DNA from the dam’s nasal discharge was positive for M. bovis by the RD4-based PCR assay; however, spoligotyping was not sensitivity enough to confirm the pattern.