Statistical analysis
We performed Gaussian Type II regression models with the standardized
major axis method to determine the relationship between the log10 eDNA
abundances obtained from qSeq and dPCR analyses with the ”sma” function
of the ”smatr” ver. 3.4.8 package in R ver. 3.6.0 (R Core Team, 2019).
For the Gaussian Type II regression model, we ignored the zero values
for the modelling. We employed the Gaussian Type II model because our
preliminary evaluation found higher R2 values for Type II regression
models with a Gaussian distribution than with a logarithmic distribution
in all cases. We compared the differences in the coefficient values by
overlapping the 95% confidence interval (CI) ranges.