Figure Legends
Figure 1. Influence of BMI on fasting C3, C3adesArg and TCC
concentrations in PCOS and control subjects (cohort 1).
Boxplots of C3 (A), C3adesArg (B) and TCC levels in PCOS subjects and
metabolically healthy controls. Data grouped according to BMI (lean: BMI
18.5-24.99; overweight: BMI 25-29.99 and obese: BMI≥30
kg/m2). Middle lines show the medians of the data and
the boxes highlight the 25th and
75th percentiles (Quarter 1 and Quarter 3). C3 levels
were significantly higher in obese subjects with PCOS compared to obese
controls (mean difference ± SEM; 0.222 ± 0.099 g/l, p < 0.05).
TCC: terminal complement complex.
Figure 2. Effects of
postprandial lipaemia on triglyceride and complement concentrations in
PCOS and control subjects.
Postprandial triglyceride (A), factor H (B), TCC (C), C3 (D), C4 (E),
properdin (F) and C3adesArg (G) levels in response to OFTT in PCOS
cohort and control groups (cohort 2). Data are mean ± SEM. Significant
differences between baseline and postprandial circulating levels in PCOS
or control groups are represented by asterisk or open circle,
respectively. Significant differences between PCOS and control groups
are denoted by the hash symbol. In all cases *, o,# represent p< 0.05; **, o
o, # # represent p< 0.001 and ***,o o o, # # # represent
p< 0.0001.
Figure 3. Influence of obesity on factor H, C3 and TCC
responses to postprandial lipaemia.
Postprandial factor H (A), TCC (B) and C3 (C) responses to OFTT in obese
(BMI ≥ 30 kg/m2) and non-obese (BMI < 30
kg/m2) PCOS and control subjects (cohort 2).
Significant differences between obese and non-obese PCOS subjects are
denoted by an asterisk (* p< 0.05; ** p< 0.001; ***
p< 0.0001). No significant differences were observed between
obese and non-obese controls.
Figure S1. Relationship between factor H area under the curve
(AUC), body fat and metabolic parameters.
Figure S2. Correlation between fasting factor H concentrations
and BMI. Data represent correlations between factor H levels and BMI in
different age groups in PCOS subjects (triangles, dashed line) and
controls (circles, solid line) (cohort 2). Factor H levels correlated
positively with BMI in both PCOS and controls in all age groups.
Table 1. Fasting plasma complement levels in cohort 1.