Figure.1 The effect of CaNAC2c silencing in pepper plants
on thermotolerance. A. Schematic diagram of the fragments used for VIGS
vectors construction, a specific fragment within the CDS
(TRV:CaNAC2c-CDS ) and a fragment within
3’UTR(TRV:CaNAC2c-3’UTR ) were used for vectors construction. B.
The success of CaNAC2c silencing by VIGS by measuring the
transcript level of CaNAC2c in HTS challenged
TRV:CaNAC2c-CDS and TRV:CaNAC2c-3’UTR pepper plants at 3
and 24 hpt.C.CaNAC2csilenced pepper plants displayed significantly decreased basal(BT) and
acquired (AT) thermotolerance(pretreated with 37℃ for 12 h and recovered
for 12 h).D. Dynamic plant mortality under HTS from 1 to 42 hpt. E-G.
The transcript level of thermotolerance related marker genes inCaNAC2c silenced pepper plants challenged with HTS at 3 and 24
hpt. H. ion leakage displayed by conductivity in CaNAC2c silenced pepper
plants pretreated with nonlethal high temperature (37 ℃ for 12 h, and
recovered for 12 h). I. ion leakage displayed by conductivity in CaNAC2c
silenced pepper plants without pretreatment with nonlethal high
temperature stress. J. H2O2 accumulation displayed by DAB staining of
different organs in CaNAC2c silenced pepper plants challenged with HTS.
K. ROS accumulation displayed by NBT staining of leaves in CaNAC2c
silenced pepper plants challenged with HTS. L. Fv/Fm and △F/Fm’ in
leaves of CaNAC2c silenced pepper plants challenged with HTS. M. Fv/Fm
and △F/Fm’ in leaves of CaNAC2c silenced pepper plants challenged with
HTS by dipping half of the leaf to water of 42℃. Data presented are
means ± standard error (SE) of four replicates. In B, E, F, G, L and M,
different uppercase letters above the bars indicated significant
differences among means (P < 0.01), as calculated with
Fisher’s protected LSD test.