Figure.4 CaNAC2c overexpressing N. benthamianaplants exhibited enhanced resistance to HTS and
RSI. A. The transcript level ofCaNAC2c in plants of two T2 transgenic N. benthamianalines. B. CaNAC2c overexpressing N. benthamiana plants displayed
significantly increased basal (BT) and acquired thermotolerance (AT)
(pretreated with 37 ℃ for 12 h and then recovered 12 h). C. The plant
mortality of the two CaNAC2c overexpressing N. benthamianalines, CaNAC2c-OE1 and CaNAC2c-OE2 under challenge of HTS
with or without pretreatment of nonlethal high temperature stress. D.
Thermotolerance related NtAPX , NtHSP18 and NtsHSPwere transcriptionally upregulatd by overexpression of CaNAC2c inN. benthamiana plants challenged by HTS. E. The resistance ofCaNAC2c overexpressing N. benthamiana plants to RSI was
significantly higher than the control plants. F. CaNAC2coverexpressing N. benthamiana plants exhibited lower dynamic
disease index than the control plants. G. The R.solanacearuminoculated CaNAC2c overexpressing N. benthamiana plants
supported lower population of R.solanacearum compared to the
control plants. In A, D, F, and G data presented are means ± standard
error (SE) of twelve and three replicates, respectively. In A, D and G
different uppercase letters above the bars indicate significant
differences among means (P < 0.01), as calculated with
Fisher’s protected LSD test.