CaNAC2c is a nuclear protein
To assay the subcellular localization of CaNAC2c, it was transiently overexpressed in N.benthamiana leaves by agro-infiltration with GV3101 cells containing 35S:CaNAC2c-GFP (using 35S:GFP as a control) and its location was assayed by GFP signal observation. The result showed that, at 48 hpi, GFP signal exclusively occurred in the nucleus of epidermal cells in leaves infiltrated with35S:CaNAC2c-GFP , while that with 35S:GFP was observed in the whole epidermal cell including plasma membrane, cytosol, and the nucleus, indicating that CaNAC2c is a nuclear protein(Figure S4).