The response of pepper NAC2Cgenesto attack of HTS and RSI
It was previously found that NAC TFs that exhibit inducible expression upon heat stress or pathogen generally play roles during plant response to these stresses(Nakashima et al., 2012). Based on the public annotated information of pepper genome, we found that there are 90 NAC transcription factors in genome of pepper lineZunla-1 (Qin et al., 2014).To find pepper NACs involved in coordination of pepper response to HTS and RSI, we identified NAC genes upregulates in pepper plants against HTS or RSI based on the public available data in Pepper Hub (http://www.hnivr.org/)(F. Liu et al., 2017) and our unpublished data from RNA-seq assay, we found that a NAC gene (Capana06g001739) that exhibits the highest sequence identity to NAC2 in Arabidopsis was upregulated by both HTS and RSI (Figure S1A and Table S3). In addition, this NAC gene was promoted by exogenous application of ABA or JA but inhibited by IAA or GA3(Figure S1B). To confirm the transcriptional response of this NAC gene to HTS or RSI, its transcript level in roots of pepper plants challenged with RSI or HTS were measured by qRT-PCR, the result showed that it was upregulated by HTS or RSI (Figure S1C).