Chromatin immunoprecipitation (ChIP) assay
ChIP assays were performed according to the protocol of previous study
Khan et al. (2018). Three fully extended pepper leaves at six-leaf stage
were inoculated with GV3101 cells harboring 35S:CaNAC2c-GFP . The
infiltrated leaves were harvested at 48 hpi and crosslinked with 1% of
formaldehyde; chromatins were isolated and sheared into fragments of 300
to 500 bp length; and DNA-protein complexes were immunoprecipitated
using anti-GFP antibodies. Finally, the DNAs were decrosslinked,
purified and used as template for PCR using primer pairs specific to a
CATGTG-box containing fragment or CATGTG free fragment in CaHSFA5promoter by semiquantitative PCR using specific primer pairs
(Supplemental Table S1).