CaNAC2c is a nuclear protein
To assay the subcellular localization of CaNAC2c, it was transiently
overexpressed in N.benthamiana leaves by agro-infiltration with
GV3101 cells containing 35S:CaNAC2c-GFP (using 35S:GFP as
a control) and its location was assayed by GFP signal observation. The
result showed that, at 48 hpi, GFP signal exclusively occurred in the
nucleus of epidermal cells in leaves infiltrated
with35S:CaNAC2c-GFP , while that with 35S:GFP was observed
in the whole epidermal cell including plasma membrane, cytosol, and the
nucleus, indicating that CaNAC2c is a nuclear protein(Figure S4).