Chromatin immunoprecipitation (ChIP) assay
ChIP assays were performed according to the protocol of previous study Khan et al. (2018). Three fully extended pepper leaves at six-leaf stage were inoculated with GV3101 cells harboring 35S:CaNAC2c-GFP . The infiltrated leaves were harvested at 48 hpi and crosslinked with 1% of formaldehyde; chromatins were isolated and sheared into fragments of 300 to 500 bp length; and DNA-protein complexes were immunoprecipitated using anti-GFP antibodies. Finally, the DNAs were decrosslinked, purified and used as template for PCR using primer pairs specific to a CATGTG-box containing fragment or CATGTG free fragment in CaHSFA5promoter by semiquantitative PCR using specific primer pairs (Supplemental Table S1).