Figure.6 The silencing of CaHSFA5 decreased
thermotolerance of pepper plants but did not significantly affect the
resistance of pepper plants to RSI. A. Schematic diagram of the
fragments in the CDS or 3’UTR of CaHSFA5 used for VIGS vectors
(TRV:CaHSFA5-CDS and TRV:CaHSFA5-3’UTR ) construction. B.
The silencing of CaHSFA5 decreased basal and acquired
thermotolerance of pepper plants. C. The silencing of CaHSFA5decreased basal and acquired thermotolerance of pepper plants. D. The
silencing of CaHSFA5 decreased Fv/Fm of pepper leaves upon HTS.E.
The silencing of CaHSFA5 decreased △F/Fm of pepper leaves upon
HTS. F. The silencing of CaHSFA5 did not affect the resistance of
pepper plants to RSI. G. The silencing of CaHSFA5 by VIGS did not
affect disease index of pepper plants upon RSI. H. The expression levels
of CaHSFA5 and CaNAC2c showed that silencingCaHSFA5 and overexpressing CaNAC2c were successful.
I.CaHSFA5 silencing significantly decreased the expression of
thermotolerance related CaHSP24 and CaHSP70 in pepper
plants. J. HR mimic cell death induced by CaNAC2c-TO at room
temperature was not affected by the silence of CaHSFA5 , andCaNAC2c-TO did not induce cell death at 37 ℃. K. CaHSFA5did not affect the expression of CaDEF1 promoted by CaNAC2c. Data
presented are means ± standard error of four replicates. In C, the data
were counted based on 12 pepper plants. In C, E, G, H, I and K, data
presented are means ± standard error (SE) of four replicates, different
uppercase letters above the bars indicated significant differences among
means (P < 0.01), as calculated with Fisher’s protected
LSD test.