The sequence analysis of pepper NAC2 genes
By sequence analysis and genome wide assay, the HTS and RSI responsive
NAC and the other three NACs all contain a well conserved N-terminal NAC
domain and a diversified C-terminal transcription regulatory (TR) region
(Figure S2A) and share the highest sequence identity to NAC2 in
Arabidopsis (Figure S2B), so they were named as CaNAC2a toCaNAC2d , and the HTS and RSI responsive NAC gene was namedCaNAC2c . Among these four genes, CaNAC2c andCaNAC2d share the highest sequence identity (Figure S2C and D).
In addition, the response of the four NAC2 genes to HTS and RSI was
assayed based on the data of RNA-seq with pepper plants challenged with
RSI, CaNAC2c is was found to be upregulated by HTS and RSI,CaNAC2d was only induced by RSI in pepper roots (Figure S2E).