Figure.4 CaNAC2c overexpressing N. benthamianaplants exhibited enhanced resistance to HTS and RSI. A. The transcript level ofCaNAC2c in plants of two T2 transgenic N. benthamianalines. B. CaNAC2c overexpressing N. benthamiana plants displayed significantly increased basal (BT) and acquired thermotolerance (AT) (pretreated with 37 ℃ for 12 h and then recovered 12 h). C. The plant mortality of the two CaNAC2c overexpressing N. benthamianalines, CaNAC2c-OE1 and CaNAC2c-OE2 under challenge of HTS with or without pretreatment of nonlethal high temperature stress. D. Thermotolerance related NtAPX , NtHSP18 and NtsHSPwere transcriptionally upregulatd by overexpression of CaNAC2c inN. benthamiana plants challenged by HTS. E. The resistance ofCaNAC2c overexpressing N. benthamiana plants to RSI was significantly higher than the control plants. F. CaNAC2coverexpressing N. benthamiana plants exhibited lower dynamic disease index than the control plants. G. The R.solanacearuminoculated CaNAC2c overexpressing N. benthamiana plants supported lower population of R.solanacearum compared to the control plants. In A, D, F, and G data presented are means ± standard error (SE) of twelve and three replicates, respectively. In A, D and G different uppercase letters above the bars indicate significant differences among means (P < 0.01), as calculated with Fisher’s protected LSD test.