Isothermal titration calorimetry (ITC)
Flag-ARWT and Flag-AR∆DBD was expressed and purified (Zhou, Suino-Powell, Ludidi, McDonnell & Xu, 2010), along with the removal of the Flag tag (Skala, Goettig & Brandstetter, 2013). Then the binding affinity between SBF-1 and both isoforms was checked using MicroCal ITC 200 (Duff, Grubbs & Howell, 2011).