Apoptosis, cell cycle and cell adhesion assay
Cell apoptosis was determined by annexin V/PI staining. In brief, the
cells were measured by flow cytometry after addition of FITC-conjugated
annexin V and PI as previously described (Azeem et al., 2017). Annexin
V+ / PI- and annexin
V+ / PI+ were considered as
apoptotic cells in the early and late phase, respectively. Samples were
analyzed by flow cytometry on a FACScan (Becton Dickinson).
For cell cycle analysis, cells were stained with propidium iodide (PI)
as described in (Sun et al., 2010b), and the cell cycle distribution was
analyzed by flow cytometry on a FACScan (Becton Dickinson). The
percentages of cells in G0/G1, S, and G2 phases were counted and
compared.
Cell adhesion assay was performed as reported (Li et al., 2012).