Cell culture
LNCaP cells expressing AR with a novel mutation T878A in the AR ligand domain, PC3/AR+ cells, a stable expressing ARWT cell line originally as a sub-line from the parental cells PC3, and human embryonic kidney cell line HEK293T were obtained from the Chinese Academy of Medical Sciences (Tianjin, China). All the cells were maintained in DMEM medium (Life Technologies, Grand Island, NY) supplemented with 10% fetal bovine serum (FBS, Life Technologies), 100 U/ml penicillin, and 100 mg/ml streptomycin.