SBF-1 showed a strong cytotoxicity against both ARWT and ARmutant prostate cancer cells
LNCaP cells (expressing AR with a novel mutation T878A in the AR ligand domain) and PC3/AR+ (PC3 cells with stable ARWT expression) were incubated with different concentrations of SBF-1 for 3 h. As the result, SBF-1 showed very strong cytotoxicity towards LNCaP and PC3/AR+ cells (Fig. 1B with IC50 values).
After incubated with different doses of SBF1, cells were collected and subjected to analysis of adhesive ability to fibronectin and laminin. SBF-1 showed a significant inhibition on adhesive ability of LNCaP and PC3/AR+ cells to fibronectin and laminin in a concentration-dependent manner (Fig. 1C).
Next, the apoptosis assay and cycle arrest analysis were shown in Fig. 1D-G. As compared with the control, the percentage of apoptotic cells was significantly increased, and the cell cycle arrest was found in G1 and G2/M phase in SBF-1-treated LNCaP and PC3/AR+cells