Phagocytosis assay
Latex beads (L3030, Sigma, St. Louis, USA) were used to detect the
potential influence of LILRA3 on the phagocytosis capacity of U937
cells. U937 cell lines harboring pWSLV-02-LILRA3 or pWSLV-02 were seeded
in 6-well plates (1.0×105cells/well) in RPMI-1640
medium containing 1% FBS, followed by incubation at 37°C in humidified
5% CO2 for 20 hours. A mixture of FBS and latex beads
(FBS: latex beads = 20:1) was added to each well. The percentage of
GFP+ Texas Red+ cells was then
analyzed by FCM at 0.5, 6, 12 and 24 hours.