Conservation analyses
The PETase-profile HMM was applied for a standard numbering scheme, by
aligning the 2930 sequences of PETase homologues from the LED against
the respective profile HMM and subsequently assigning the position
numbers from the Is PETase reference sequence as standard
position numbers. For conservation analysis of PETase homologues, the
frequency of amino acid residues or gaps was counted at each standard
position.
For the conservation analysis of PURase homologues from LED
superfamilies 11 and 13, two multiple sequence alignments were generated
using Clustal Omega (version 1.2.4) 23, and the
frequency of amino acid residues or gaps was counted at selected
positions.