Animal experiment
Male C57BL/6J mice (8- to 9-week-old) were obtained from Laboratory
Animal Center of Sun Yat-sen University (Guangzhou, China).
Liver-specific YAP-deficient mice and paired WT mice were generated by
Shanghai Model organisms Center, Inc (Shanghai, China). Male C57BL/6J
mice were i.p. injected with vehicle (Corn oil, Aladdin, C116025)
or 3 mg/kg/d TCPOBOP (Sigma-Aldrich, T1443, St. Louis, MO). Liver tissue
and serum samples were harvested after 5 and 10 days. WT mice werei.p. injected with vehicle or 3 mg/kg/d TCPOBOP following PHx.
Tissue and serum samples were collected at 2 and 5 days after the
surgery. Male WT and Yap -/- mice (8- to
9-week-old) were i.p. injected with vehicle (Corn oil) or 3
mg/kg/d TCPOBOP and the tissue samples were collected at day 10. Serum
and liver samples were snap frozen in liquid nitrogen, then stored at
-80℃ for further use. A portion of liver was immediately fixed in 10%
formalin for histological section. The animal treatments were approved
by the Institutional Animal Care and Use Committee at Sun Yat-sen
University, Guangzhou, China. The entire study stuck to the 3Rs
principle of animal experiments, under the guideline of ARRIVE
guidelines with respect.