Quantitative real-time PCR analysis
Total RNA from liver tissue samples were extracted by using Trizol reagent. Single-strand cDNA was synthesized by reverse transcription of total RNA using Primer Script RT reagent Kit (Takara, Japan). All sequence of primers used for quantitative RNA analysis were listed in Supplementary information. The mRNAs were amplified in Biosystems 7500 Real-time PCR reaction system using SYBR Premix Ex-Taq II Kit (Takara, Japan) according to the manufactory’s instructions. The fold changes of mRNA levels were analyzed by δδCt method.