Other factors are involved in CAR-induced hepatomegaly.
To investigate other possible factors involved in CAR-induced liver enlargement, the mRNA levels of other related genes such asC-myc , Foxm1 , Ctnnb1 , Egfr and Metwere measured in TCPOBOP-treated WT mice andYap -/- mice (Figure 5A and 5B). The results suggested that C-myc , Foxm1 , Ctnnb1 and Metwere significantly upregulated in TCPOBOP-treated WT mice and TCPOBOP-treated Yap-/- mice. Then the protein levels of C-Myc, FOXM1, CTNNB1 and MET were measured and the protein levels of C-Myc, CTNNB1 and MET were significantly upregulated in TCPOBOP-treated WT mice andYap -/- mice, indicating that these signaling pathways also contribute to CAR-induced liver enlargement whenYap was absent (Figure 5C and 5D). These data suggested that many different pathways were involved in CAR -induced hepatomegaly, the knockout of Yap only relieve the liver enlargement to some extent but cannot totally eliminate the proliferation effect caused by other pathways such as C-Myc, CTNNB1 and MET signaling.