Co-immunoprecipitation (Co-IP)
HepG2 cells (ATCC, Virginia, USA) were cultured in DMEM containing 10 %
FBS. Co-immunoprecipitation (Co-IP) was performed using Thermo
Scientific Pierce Co-IP kit (Thermo Scientific, 26149, Rockford, IL)
following the manufacturer’s protocol as described in our previous
report (Jiang et al., 2019). Samples were analyzed by western blotting
using anti-IgG (Abcam, ab133470), anti-CAR (Abcam, ab62590) and anti-YAP
(Cell Signaling Technology, #14074, Danvers, MA).