Animal experiment
Male C57BL/6J mice (8- to 9-week-old) were obtained from Laboratory Animal Center of Sun Yat-sen University (Guangzhou, China). Liver-specific YAP-deficient mice and paired WT mice were generated by Shanghai Model organisms Center, Inc (Shanghai, China). Male C57BL/6J mice were i.p. injected with vehicle (Corn oil, Aladdin, C116025) or 3 mg/kg/d TCPOBOP (Sigma-Aldrich, T1443, St. Louis, MO). Liver tissue and serum samples were harvested after 5 and 10 days. WT mice werei.p. injected with vehicle or 3 mg/kg/d TCPOBOP following PHx. Tissue and serum samples were collected at 2 and 5 days after the surgery. Male WT and Yap -/- mice (8- to 9-week-old) were i.p. injected with vehicle (Corn oil) or 3 mg/kg/d TCPOBOP and the tissue samples were collected at day 10. Serum and liver samples were snap frozen in liquid nitrogen, then stored at -80℃ for further use. A portion of liver was immediately fixed in 10% formalin for histological section. The animal treatments were approved by the Institutional Animal Care and Use Committee at Sun Yat-sen University, Guangzhou, China. The entire study stuck to the 3Rs principle of animal experiments, under the guideline of ARRIVE guidelines with respect.