2.3 Genome sequencing
Genomic DNA was extracted from a single adult female of S. peregrina using SDS method (Additional file 1: Table S1) (Rinkevichet al. 2006). A 20-kb insert SMRTbell library was generated using the SMRT bell Template Prep Kits according to the selection protocol on the BluePippin (Sage Science, MA, USA). SMRT sequencing was performed on PacBio Sequel instrument with 11 SMRT cells at the Genome Center of Nextomics (Wuhan, China). After removal of low quality and sequencing adapters, the clean subreads were used for subsequent genome assembly. Furthermore, in order to assist genome annotation, total RNAs were extracted from a whole single adult female using the mirVana miRNA Isolation Kit (Ambion) following the manufacturer’s protocol. RNA-seq libraries were then constructed using TruSeq RNA Library Preparation Kit (Illumina, CA, USA), and paired-end sequencing (PE 150 bp) was performed on HiSeq Xten platform. A total of 9.62 Gb of raw data was produced for assisting genome annotation (Additional file 1: Table S2).