2.3 Genome sequencing
Genomic DNA was extracted from a single adult female of S.
peregrina using SDS method (Additional file 1: Table S1) (Rinkevichet al. 2006). A 20-kb insert SMRTbell library was generated using
the SMRT bell Template Prep Kits according to the selection protocol on
the BluePippin (Sage Science, MA, USA). SMRT sequencing was performed on
PacBio Sequel instrument with 11 SMRT cells at the Genome Center of
Nextomics (Wuhan, China). After removal of low quality and sequencing
adapters, the clean subreads were used for subsequent genome assembly.
Furthermore, in order to assist genome annotation, total RNAs were
extracted from a whole single adult female using the mirVana miRNA
Isolation Kit (Ambion) following the manufacturer’s protocol. RNA-seq
libraries were then constructed using TruSeq RNA Library Preparation Kit
(Illumina, CA, USA), and paired-end sequencing (PE 150 bp) was performed
on HiSeq Xten platform. A total of 9.62 Gb of raw data was produced for
assisting genome annotation (Additional file 1: Table S2).