Figure 7. Gene-inhibiting efficiency of LP-Td-O targetinggfp in E. coli expressing GFP. a) Confocal microscopy
imaging to investigate GFP fluorescence intensity after treating
bacteria with 1 μM Td-Oanti-gfp with or without
LP2000 for 6 h. Red fluorescence was from FM4-64. b) Semiquantitative
analysis of GFP fluorescence intensity. c) Quantitative real-time PCR
analysis of gfp mRNA levels after exposure to different
concentrations of Td-Oanti-gfp or
LP-Td-Oanti-gfp (0.1, 0.5, or 1 μM) for 3 h.
Td-Omis was used as a negative control to confirm
specific gene inhibiting of ASOs. Scale bars represent 10 μm. “*”:p <0.05; “**”: p <0.01 versus control group.