Figure 8. Bacterial growth inhibitory effect of LP-Td-L by inhibiting the acpP gene in E. coli. a) Colony counting of E. coli after treatment with different concentrations of Td-Lanti-acpP (0.1, 0.5, or 1 μM) with or without LP2000 for different times (1, 3 or 5 h). b) Quantitative real-time PCR analysis of acpP mRNA levels after exposure to different concentrations of Td-Lanti-acpP or LP-Td-Lanti-acpP (0.1, 0.5, or 1 μM) for 3 h. Td-Lmis was used as a negative control to confirm specific gene inhibiting of ASOs. “*”: p <0.05; “**”:p <0.01; “***”: p <0.001 versus control group.
Supporting Information
Loop-armed DNA Tetrahedron Nanoparticles for Delivering Antisense Oligos into Bacteria
Yue Hu†, 1, Zhou Chen†, 1, Xinggang Mao‡, 1, Mingkai Li, Zheng Hou, Jingru Meng, Xiaoxing Luo†,*, Xiaoyan Xue†,*