4.2.7 Cell growth assay.
E. coli bacteria were cultured in LB medium until the early log
stage, then diluted to 1×105 CFU/mL and treated with
Td-L at 0.1, 0.5, or 1 μM for 1, 3,
or 5 h. At every time point, a 10 μL bacterial suspension was diluted
and plated. After incubating at 37 °C overnight, colony counts in every
sample were expressed as CFU/ml.