4.1 Materials
All oligonucleotides were obtained from Sangon Biotech (China).
Magnesium chloride hexahydrate was purchased from Tian Li (China).
Trizma base was obtained from Sigma-Aldrich (USA). Agarose was purchased
from Lonza (USA). DNase I was obtained from Solarbio (China).
Lipofectamine 2000 (LP2000) was purchased from Invitrogen (USA). FM4-64
was purchased from Molecular Probes
(USA). Phosphate-buffered saline (PBS) and Mueller-Hinton broth (MHB)
were purchased from Boster (China) and Land Bridge (China),
respectively. The PrimeScript RT Reagent Kit with DNA Eraser and Premix
Taq RT-PCR System were from Takara Bio Inc (Japan). The water used in
all experiments was prepared via a Millipore Milli-Q purification system
with a resistivity greater than 18 MΩ cm-1.