Figure 7. Gene-inhibiting efficiency of LP-Td-O targetinggfp in E. coli expressing GFP. a) Confocal microscopy imaging to investigate GFP fluorescence intensity after treating bacteria with 1 μM Td-Oanti-gfp with or without LP2000 for 6 h. Red fluorescence was from FM4-64. b) Semiquantitative analysis of GFP fluorescence intensity. c) Quantitative real-time PCR analysis of gfp mRNA levels after exposure to different concentrations of Td-Oanti-gfp or LP-Td-Oanti-gfp (0.1, 0.5, or 1 μM) for 3 h. Td-Omis was used as a negative control to confirm specific gene inhibiting of ASOs. Scale bars represent 10 μm. “*”:p <0.05; “**”: p <0.01 versus control group.