Experimental Approach
Different bacterial strains were incubated with Td, and the bacterial
uptake efficiency of Td was measured by flow cytometry and
confocal microscopy. Then we designed
two types of linkages modes between Td and ASOs targeting gfp ,
encoding green fluorescent protein , or acpP , encoding the acyl
carrier protein, and assessed the efficiency of delivery by flow
cytometry and gene knockdown by
RT-PCR, colony counting and confocal
microscopy in E. coli .