Key results
Td was more likely to adhere to bacterial membranes, with moderate
ability to penetrate into the bacteria. Strikingly, Lipofectamine 2000
(LP2000) in a 0.125 μL/μg ratio to Td helped Td penetrate into bacteria,
but had no apparent effect on linear DNA in the same concentration.
Furthermore, linkage modes between ASOs and Td influenced gene-knockdown
efficiency.
Looped structure of ASOs linked to one side of the Td exhibited better
gene-knockdown efficiency than the overhung structure.
Conclusion and
Implications
This study established an effective
antisense delivery system based on loop-armed Td, which opens
opportunities for developing antisense antibiotics.
Keywords : DNA tetrahedron, antisense antibiotics,
nanoparticles, drug delivery system
Bullet point summary :