4.2.8 RT-PCR
After incubation with Td, the bacteria were harvested. The total RNA of
the bacteria was extracted and then reverse-transcribed using the
PrimeScript RT Reagent Kit with DNA Eraser. Amplification was performed
according to the following steps: denaturation at 95 °C for 5 min and 40
cycles of 95 °C for 10 s and 58 °C for 30 s. gyrA was used as an
internal control.