4.2.8 RT-PCR
After incubation with Td, the bacteria were harvested. The total RNA of the bacteria was extracted and then reverse-transcribed using the PrimeScript RT Reagent Kit with DNA Eraser. Amplification was performed according to the following steps: denaturation at 95 °C for 5 min and 40 cycles of 95 °C for 10 s and 58 °C for 30 s. gyrA was used as an internal control.