Experimental Approach
Different bacterial strains were incubated with Td, and the bacterial uptake efficiency of Td was measured by flow cytometry and confocal microscopy. Then we designed two types of linkages modes between Td and ASOs targeting gfp , encoding green fluorescent protein , or acpP , encoding the acyl carrier protein, and assessed the efficiency of delivery by flow cytometry and gene knockdown by RT-PCR, colony counting and confocal microscopy in E. coli .