Key results
Td was more likely to adhere to bacterial membranes, with moderate ability to penetrate into the bacteria. Strikingly, Lipofectamine 2000 (LP2000) in a 0.125 μL/μg ratio to Td helped Td penetrate into bacteria, but had no apparent effect on linear DNA in the same concentration. Furthermore, linkage modes between ASOs and Td influenced gene-knockdown efficiency.
Looped structure of ASOs linked to one side of the Td exhibited better gene-knockdown efficiency than the overhung structure.

Conclusion and Implications

This study established an effective antisense delivery system based on loop-armed Td, which opens opportunities for developing antisense antibiotics.
Keywords : DNA tetrahedron, antisense antibiotics, nanoparticles, drug delivery system
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