Anthesis QTL not segregating for frost tolerance
No frost QTL were detected on chromosome 1B. However, anthesis and maturity QTL were present on the central chromosome regions in the G7A and SpM populations and on the distal region of 1B short arm in the SpB population (Supplementary Fig. 8). Gene TaFT3_1B (1B: 581.4 Mb) was tightly linked to SNP marker 1BM77588 (1B: 581.6 Mb), which is next to the anthesis QTL region (1BM54518) in the G7A population (Supplementary Fig. 8). In the SpM population, the TaFT3_1Blocus was possibly located above 1BM73284 (1B: 662.9Mb), on the anthesis QTL region. On the physical map, TaELF3_1B was located on the distal region of the long arm in these three populations. Two other anthesis-related genes, TaWUSCHELL_1B (1B: 53.3 Mb) andTaTOE1_1B (1B: 59.1 Mb), were close to markers M42781 (1B: 56.8 Mb) and M47932 (1B: 58.7 Mb) on the Chinese Spring physical map. These two genes were located 25 cM away from the maturity QTL in the SpB population. It is not clear whether the QTL was influenced by them.
Another chromosome without frost QTL was 5B, even though anthesis QTL are present in the St3B, SpB, Sp7A and SpM populations, andVrnB1a was segregating in the St3B, SpB and Sp7A populations (Supplementary Fig. 9). The anthesis QTL was on or closely linked to theVrn1_5B allele in the St3B and SpB populations, and to the distal regions of either the long arm in the Sp7A population or the short arm in the SpM population.