Cryo-Transmission Electron Microscope
For the cryo-TEM characterization a drop of the same PDA-PAR30 particle suspension was deposited on a carbon coated electron transmission microscopy grid. The drop was aspired with filter paper to obtain a particle containing film covering the whole carbon membrane. Those coated grids were immersed in ethane at the temperature of liquid nitrogen, transferred on the cryo-holder and inserted in the electron microscope. Analysis was performed at 200 kV with a Jeol 2100F transmission electron microscope. To reduce the irradiation damage, the images were acquired using a low-density electron beam.