2.2.1 Synthesis of polydopamine nanoparticles (PDA NPs) and PDA
NPs decorated with PAR30 (PDA-PAR30)
PAR30 powder was dissolved in TRIS buffer 50 mM at pH =
8.5 at a concentration of 1 mg.mL-1. Then the
PAR30 solution was mixed with dopamine powder in order
to obtain a concentration of 0.2 mg.mL-1. This
solution was stirred for 24 hours under stirring (300 RPM) (Scheme 1).
After that, 7 steps of dialysis in 1000 mL TRIS buffer were performed to
remove the PAR30 non deposited onto PDA NPs. Final
solution was stored at +4°C. PDA NPs were synthesized using the same
protocol just by removing PAR30 deposition step.