Cryo-Transmission Electron Microscope
For the cryo-TEM characterization a drop of the same
PDA-PAR30 particle suspension was deposited on a carbon
coated electron transmission microscopy grid. The drop was aspired with
filter paper to obtain a particle containing film covering the whole
carbon membrane. Those coated grids were immersed in ethane at the
temperature of liquid nitrogen, transferred on the cryo-holder and
inserted in the electron microscope. Analysis was performed at 200 kV
with a Jeol 2100F transmission electron microscope. To reduce the
irradiation damage, the images were acquired using a low-density
electron beam.