Histochemical assay
Ten-day-old transgenic Arabidopsis plants were spread withV. dahliae conidial suspension (1×10^6conidia·ml-1), 2 μM peptides of either flg22 or nlp20,
for 12 h. Peptides of flg22 and nlp20 were synthesized in GenScript
(Nanjing, China). Plants were incubated in the GUS staining solution at
37°C for 6 h, followed by decolorization in 75% alcohol and then
photographed under stereoscopic microscopy (MZFLIII, Leica, Wetzlar,
Germany) according to Sun et al. (2014).