Figure S4: GhCSD family members positively regulate
cotton resistance to Verticillium dahliae.
A: Reverse transcription-quantitative PCR (RT-qPCR) evaluation
of the relative expression levels of GhCSD1 , GhCSD2 ,GhCSD3 and GhCCS induced by V. dahliae at different
time points, as compared with mock treatment. Values represent the means
± s. d. from three biological replicates.
B: Disease symptoms of cotton transformed with variousTRV constructs: TRV:00 , TRV:GhCSD1 ,TRV:GhCSD2 , TRV:GhCCS , TRV:NB-ARC plants 16 days
after inoculation with ‘V991’. Photos were obtained 16 days after
inoculation.
C: Relative expression levels of the corresponding genes in
root samples from TRV:00 and TRV:target-genes plants
RT-qPCR.
D: Disease index statistics of TRV:00 and TRV:target-genesplants at 16 days after inoculation.
Figure S5: Sub-cellular localization and Verticillium
dahliae-induced expression patterns of GhCSD1, GhCSD2,GhCSD3, and GhCCS. Transient expression of green
fluorescent protein (GFP) or the GhCSD1-GFP, GhCSD2-GFP, GhCSD3-GFP,
GhCCS-GFP fusion proteins in tobacco leaf cells. The green color
indicates GFP expression, and red color indicates chloroplast
autofluorescence, Bars= 20 μm.
Figure S6: Overexpression of miR398b and GhrCSD2 affect
the expression levels of other SOD members in cotton upon NLP
treatments. Reverse transcription-quantitative qPCR (RT-qPCR) analysis
showing the relative expression patterns of GhMSD1 ,GhFSD2 , GhCSD1 , GhCCS , GhRbohB , and5NB in wild-type (WT) and transgenic plants upon NLP treatment.
Figure S7: Co-expression networks of the miR398b target modules
in cotton. Diagram was drawn using ccNET (structural
biology.cau.edu.cn). Gh_D09G0858, Gh_D05G3257 and Gh_A04G0380 are
cotton gene IDs for GhCSD2 , 5NB and 4NB ,
respectively.