Plant materials and V. dahliae proliferation
Seedlings of G. hirsutum cv . YZ1, G. barbadense cv .
7124 and transgenic lines derived from G. hirsutum cv .
J668 were grown
in
a controlled environment chamber under a 16 h light/8 h dark cycle at
25°C for treatment and sampling. V. dahliae strain V991 was
cultured on Potato Dextrose Agar (PDA) medium for 3~4
days, and hyphae were transferred into
Czapek’s
medium for 3 days at 25°C to prepare suspension solutions.