3.2 Promoter analysis of PpINHs
VIN regulates sucrose metabolism in conjunction with INH interaction proteins to enable plants to resist cold stress. To determine whetherPpINH gene expression is sensitive to low temperature,PpINH promoters (defined as regions 3000 bp in length directly upstream from PpINH coding sequences) were examined in the peach genome. CBF/DREB (C-repeat binding factor/dehydration responsive element) binding sites occur within each PpINH promoter(Figure 2). The CBF/DREB protein binds specifically to G(TG)CGG cis-elements involved in regulating plant responses to drought, low temperature, and stress. These results support the hypothesis that INH proteins act as key regulators in flowering plants to mediate the response to cold stress, especially in dicots.