2.7 Quantitative real time PCR (qRT-PCR) analysis
RNA isolation and cDNA synthesis were performed according to He et al. (2018). The primers used for qRT-PCR are shown in Table S1 . Translation elongation factor 2 (TEF2, JQ732180.1) was chosen as a reference gene (Wang et al., 2013). Other experimental methods related to qRT-PCR were as described by He et al. (2018).