PLBD2 in Drug Substance
It has been reported that PLBD2 proenzyme (MW 64 kDa) was able to
undergo limited autolysis leading to the formation of a 28 kDa
N-terminal prodomain and a 40 kDa C-terminal mature protein (Deuschl,
Kollmann, von Figura, & Lubke, 2006)’ (Lakomek et al.,
2009). Three different forms of PLBD2 at MW of 64 kDa, 40 kDa and 28 kDa
were all observed in the drug substance using Western blot (Figure 2,
lane 2). Recombinant CHO PLBD2 also contained the three different
molecular weight species (Figure 2, lane 5). Interestingly, recombinant
human PLBD2 purchased from OriGene contained only proenzyme at 66 kDa
(Figure 2, lane 4).